A New Natural Naphthoquinone in Mycobacterium phlei

Genetic transfer in Mycobacterium phlei.

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

Citrate formation in Mycobacterium phlei.

Although it is well known that citrate stimulates the growth of acid fast bacteria, the metabolic role of citrate is still obscure in this bacterial group. Washed suspensions of mycobacteria either fail to oxidize citrate or oxidize this substance slowly (Edson, 1951). On the other hand, clarified extracts of mycobacteria oxidize citrate to a-ketoglutarate (Geronimus, Gray, and Birkeland, 1949;...

The Mycobacterium phlei Genome: Expectations and Surprises

Mycobacterium phlei, a nontuberculosis mycobacterial species, was first described in 1898-1899. We present the complete genome sequence for theM. phlei CCUG21000(T)type strain and the draft genomes for four additional strains. The genome size for all five is 5.3 Mb with 69.4% Guanine-Cytosine content. This is ≈0.35 Mbp smaller than the previously reported M. phlei RIVM draft genome. The size di...

“Mycobacterium massilipolynesiensis” sp. nov., a rapidly-growing mycobacterium of medical interest related to Mycobacterium phlei

In French Polynesia, respiratory tract clinical isolate M26, displayed unusual phenotype and contradictory phylogenetic affiliations, suggesting a hitherto unidentified rapidly-growing Mycobacterium species. The phenotype of strain M26 was further characterized and its genome sequenced. Strain M26 genome consists in a 5,732,017-bp circular chromosome with a G + C% of 67.54%, comprising 5,500 pr...